Ocean Carbon and Biogeochemistry Data System

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EDDIES cruise: 2004 and 2005 R/V Oceanus cruises
HPLC pigments processing notes

PI: Dennis McGillicuddy (WHOI)

02 November 2007: Prepared for OCB data system by Nancy Copley (Biology Dept, WHOI) and Cyndy Chandler, BCO-DMO (MCG Dept, WHOI).

Methodology: NASA/TM-2003- HPLC Protocols (1 MB PDF) J.L. Mueller, G.S. Fargion, C.R. McClain, C.C. Trees and J. Perl. 2003. "Ocean Optics Protocols For Satellite Ocean Color Sensor Validation, Revision 5, Volume V: Biogeochemical and Bio-Optical Measurements and Data Analysis Protocols". NASA Tech. Memo., NASA Goddard Space Flight Center, Greenbelt, Maryland. 43 pp. (PDF downlaoded from http://oceancolor.gsfc.nasa.gov/DOCS/)

Technician: Jason Perl (jperl@chors.sdsu.edu)
Center for Hydro-Optics & Remote Sensing
San Diego State University Research Foundation

Technician notes:
chl_a_fluor and pheo_fluor are values from a Turner Fluorometer. Each HPLC extract is run on the Fluorometer as a second measure of Chla. Chla, MVa, DVa using the C18 HPLC method, yield a single peak for Chla, measured at 436nm and 450nm. A regression equation, between the 2 wavelengths (based on calibration curves of 100% MVa and 100% DVa), is used to give values for each pigment within a single peak.